1. SADIA QAMAR ARAIN - Senior Lecturer, Department of Biochemistry, The University of Modern Sciences, Tando Muhammad
Khan. Sindh Pakistan, Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan, National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro -76080, Pakistan & Department of Biochemistry, Beachwood Institute of Nursing, Hyderabad.
2. FARAH NAZ TALPUR - National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro -76080, Pakistan.
3. NASEEM ASLAM CHANNA - Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan.
4. NAZAKAT HUSSAIN MEMON - Department of Biochemistry, Ghulam Muhammad Mahar Medical College and Hospital Sukkur, Shaheed
Mohtarma Benazir Bhutto Medical University Larkana, Sindh, Pakistan.
5. MARVI SHAIKH - Senior Lecturer, Department of Biochemistry, The University of Modern Sciences, Tando Muhammad
Khan. Sindh Pakistan & Institute of Biochemistry, University of Sindh, Jamshoro, Pakistan.
6. UMEDAN CHANNA - National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro -76080, Pakistan.
7. ARSHAD HUSSAIN LAGHARI - Department of Biochemistry, Ghulam Muhammad Mahar Medical College and Hospital Sukkur, Shaheed
Mohtarma Benazir Bhutto Medical University Larkana, Sindh, Pakistan.
Background: Cirrhosis is a liver disease in which irreversible scarring of the hepatic cells occurs that alters the lipid metabolism. The purpose of the current study was to ascertain metabolic alteration that occurred in hepatitis C cirrhosis patients.
Methodology: The blood samples of hepatitis C cirrhosis patients and controls were collected in a fasting condition. Serum lipid analysis was done by gas chromatography and micro-lab. The data were analyzed by using SPSS 15 and SAS statistical software.
Results: The MELD (model for end-stage liver disease) score showed the liver function; AST to APRI (platelet ratio index) and FIB-4 (fibrosis-4) score indicated the low degree of fibrosis in the patients. Hypolipidemia with a reduced level of triglyceride, VLDL-C, LDL-C, HDL-C, and cholesterol was observed in hepatitis C cirrhosis patients. The hepatitis C cirrhosis patients showed elevated quantities (p<0.05) of palmitoleic and palmitic acids while reduced concentrations (p<0.05) of linoleic acid (LA), docosenoic, arachidonic, and eicosapentaenoic (EPA) acids were detected in comparison to control subjects. The lignoceric, arachidic, myristoleic, nervonic, α-linolenic acid (ALA), docosapentaenoic acid (DPA), and eicosatrienoic (ETE) acids were not detected in patients. The SFA and MUFA were comparatively augmented (p<0.05), and PUFA was reduced in the serum of hepatitis C cirrhosis patients compared with controls.
Conclusion: Hepatitis C cirrhosis patients were found with a lower degree of fibrosis and augmented concentrations of SFA and MUFA, while reduced PUFA levels may be possibly due to altered desaturase activities, non-enzymatic oxidation reaction, and impaired PUFA metabolism.
Fatty acids, cholesterol, GC-FID, lipotoxicity, lipogenesis, Triglycerides.