Manuscript Title:

RESPONSE SURFACE METHODOLOGY FOR THE EXTRACTION OF POLYPHENOL CONTENTS AND HPLC PROFILING OF CUCUMIS SATIVUS PEELS

Author:

FAKHAR ABBAS, AYOUB RASHID CHAUDHRY, MUHAMMAD IMRAN UL HAQ, HAFIZ ABDUL BASIT MUHAMMAD, KHALID HAMID, MUHAMMAD WASEEM IQBAL, SALMAN UBAID BHATTI

DOI Number:

DOI:10.17605/OSF.IO/4YFET

Published : 2022-10-10

About the author(s)

1. FAKHAR ABBAS - PhD Scholar, Department of Chemistry, GCU Lahore.
2. AYOUB RASHID CHAUDHRY - Associate Professor Department of Chemistry GCU Lahore.
3. MUHAMMAD IMRAN UL HAQ - PhD Scholar, Department of Chemistry, GCU Lahore.
4. HAFIZ ABDUL BASIT MUHAMMAD - PhD Scholar, Department of Computer Science, Superior University Lahore.
5. KHALID HAMID - PhD Scholar Department of Computer Science, Superior University Lahore.
6. MUHAMMAD WASEEM IQBAL - PhD Scholar, Department of chemistry, GCU Lahore.
7. SALMAN UBAID BHATTI - Lecturer Department of Computer Science, SABAC College Lahore.

Full Text : PDF

Abstract

Response surface methodology was used to optimize the extraction yield of total polyphenol contents from the Curcumis sativus peels. The experiment design software 13.01 was used for the optimization by selecting three independent design variables; solvent composition (40-100%), extraction temperature (21- 54 ºC) and extraction time (17-43, minutes). The most suitable extraction conditions evaluated using mathematical model were at 70% methanol composition, 37º C extraction temperature and 30 minutes time. The extraction rate of TPC (Total Phenolic Content) was 22.055 at these optimal conditions. The profiling of methanolic peel extract at these optimized conditions was carried out by HPLC analysis. The compounds identified by HPLC included caffeic acid, gallic acid, protocatechuic acid, kampferol, chlorogenic acid and vannilic acid. Abbreviations: DPPH (2, 2-diphenyl-1-picrylhydrazyl), RSM (Response surface methodology), HPLC (High performance liquid chromatography), ROS (reactive oxygen species), ND (not detected).


Keywords

Cucumis sativus, Lipase inhibition, ROS, Antioxidant potential, HPLC, Glucosidase inhibition, RSM.